Characterization & Partial purification of Microbial Lipase enzyme produce by Serratia nematodiphilia isolated from Paper & pulp effluent

Abstract

As the demand is growing for paper and pulp at a highest peak, it not only imposes a severe threat on the forest resources but also on the environment. The effluent discharged by these paper and pulp industries is of great concern. Along with the biodegradation of theses effluents, we also aimed to produce microbial lipase. Lipases, a class of enzymes which catalyze the hydrolysis of long chain triglycerides. At present, microbial lipases are attaining much awareness with the rapid progress of enzyme technology. Lipases represent the most significant group of biocatalysts for industrial applications. In the present study various samples were collected from the paper and pulp effluent from Vapi, Gujarat. For preliminary understanding, total 18 bacterial isolates (JKB1 to JKB18) and 5 Fungi isolates (S1 to S5) were isolated out of that. Based on their primary screening technique, JKB1, JKB3, JKB5 and JKB21 bacterial isolates were studied for further for lipase production. Results show that isolate JKB3 was more potential and was studied further for characterization of lipase enzyme. It was identified as Serratia nematodaphilia. The results also indicate that the maximum activity was obtained i.e. 59.66 U/ml in optimized conditions like Glucose 2.75 U/ml, Tryptone 32.39 U/ml, Peanut oil 34.71U/ml, pH-7 64.92 U/ml, room temperature. At the end of partial purification, 5.15 fold purification of lipase was obtained. The current study represents the isolation and characterization of lipase enzyme which has a great applicability in paper & pulp effluent in deinking process in making of paper and increasing brightness, intensity and the pulping rate. It can also decrease chemical consumption, prolong machinery life, reduction in pollution, save energy, time and production cost.