Nephroprotective effect of Aqueous Extract of the Hordeum vulgare L. in wistar rats intoxicated by Aluminium chloride

Fidah Hadj; Hachem Kadda; Adli Djallal E.H.; Brahmi Mostapha; Merad Zakaria; Kahloula Khaled; Slimani Miloud

doi:10.38150/sajeb.10(1).p44-51

Authors

Fidah Hadj Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants, Department of Biology, Faculty of Sciences, University of Dr Moulay Tahar, Saida, Algeria
Hachem Kadda Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants, Department of Biology, Faculty of Sciences, University of Dr Moulay Tahar, Saida, Algeria
Adli Djallal E.H. Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants, Department of Biology, Faculty of Sciences, University of Dr Moulay Tahar, Saida, Algeria
Brahmi Mostapha Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants, Department of Biology, Faculty of Sciences, University of Dr Moulay Tahar, Saida, Algeria
Merad Zakaria Department of Pathological Anatomy and Cytology, CHU of Sidi Bel Abbes, Algeria
Kahloula Khaled Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants, Department of Biology, Faculty of Sciences, University of Dr Moulay Tahar, Saida, Algeria
Slimani Miloud Laboratory of Biotoxicology, Pharmacognosy and Biological Recovery of Plants, Department of Biology, Faculty of Sciences, University of Dr Moulay Tahar, Saida, Algeria

DOI:

https://doi.org/10.38150/sajeb.10(1).p44-51

Abstract

The present study was undertaken to determine the effectiveness of the extract of Hordeum vulgare L to restore the adverse effects induced by the aluminum chloride (AlCl3) in Wistar rats, and this is through gavage with a dose of 13 ml/kg/d during a period of 21 days. H.vulgare L extraction by mac-eration allowed us to obtain an aqueous extract with a yield of 9,7%. Expo-sure to AlCl3at a concentration of 100 mg/kg in rats showed a decrease in body and kidney weight compared with those of the witness. This AlCl3 poi-soning also revealed a disruption of the various biochemical parameters, notably renal biomarkers. Indeed, the histological study showed very marked lesions in the renal tissue. In addition, the administration of aqueous extract of Hordeum vulgare L(H.V.A.E)resulted in a regain of body and kidney weight in rats compared to intoxicated rats. The results of the renal biochemical assays (urea, uric acid and creatinine) show a correction of the values following the administration of (H.V.A.E) compared to those of the intoxicat-ed animals. The analysis of antioxidant status in the kidney indicates that (H.V.A.E) restored the activity of the different antioxidant enzymes (SOD, CATand GPx) in (H.V.A.E)-treated and intoxicated rats. In effect, the histolog-ical study undertaken has shown a marked improvement in renal tissue ar-chitecture in intoxicated rats and treated with (H.V.A.E). This confirms that (H.V.A.E) protects the kidney against the toxicity of aluminum chloride.