In vitro culture and adventitious rhizogenesis of Bambusa tulda Roxb. in agro-climatic conditions of Bihar, India

Abstract

Bambusa tulda is highly economical bamboo, playing an important role as non-timber raw material for large number of products. Forest resource of this bamboo is depleting at a high rate and hence to meet its growing demand in commercial sector, technique for industrial production is required. The present experimental studies were carried out for rapid and mass clonal propagation of B. tulda covering two major aspects of propagation i.e. estab-lishment of aseptic culture and adventitious rhizogenesis. Organogenetic mode of regeneration of plant tissue culture technique has been employed for massive production of this bamboo species. The treatment of explant with Dettol (0.4%) for 10 mins and Bavistin (0.2%) for 30 mins followed by Ethyl alcohol (70%) for 35 seconds and HgCl2 (0.1%) for 10 mins resulted with maximum number of survival of aseptic cultures. The effect of MS medium+ additives+ NAA (0.25mg/l) supplemented with TDZ (0.5mg/l) showed maxi-mum (96%) shoot initiation. Maximum root growth (8.8cm) and number (approx 7 roots/ clump) was observed in ½ strength of MS with IBA (4mg/l) supplemented with coumarin (4mg/l). Seventh sub-culture cycle/ passage was the most suitable and the earliest possible passage of cultures (sub-culturing) for generation of profused in vitro rooting. The establishment of aseptic in vitro cultures and profused adventitious root induction derived from above studies will further help in industrial production of quality planting material of B. tulda within short span of time.