SDS-PAGE protein profile of Candida albicans isolated from patients with atopic dermatitis

Abstract

Candida albicans is a dimorphic fungus that exists as a normal flora of hu-mans. It colonizes mucosal surfaces of the digestive tract, oral cavity and genitourinary tract and is also able to cause a variety of infections, depend-ing on the nature of the underlying host defect. This microorganism can ex-acerbate clinical conditions of atopic dermatitis via protein Allergens. The aims of this study were evaluation of protein profile of C. albicans in patients with atopic dermatitis. Four isolates of Candida albicans were collected from oral cavity of patients with atopic dermatitis. Proteins of Candida albicans were separated and reduced by sodium dodecylsulfate polyacrylamide gel electrophoresis. The extracts obtained from various isolates of Candida albicans had 62 protein bands, which ranged from 11 to >180 kDa. Sixteen different types of protein were identified. The maximum number of bands was observed in isolate B. The results of this study indicated that Candida albicans consisted of various somatic proteins that can trigger IgE-mediated reaction and exacerbation of atopic dermatitis.