Detection of Pan‐amino glycoside‐resistant Gram negative Bacteria using a Molecular Method

Authors

  • Lakshmana Gowda College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia
  • James John Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, Tamil Nadu, 632004, India
  • Mohammed Ali M. Marie College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia
  • Sangeetha Gopalkrishnan Microbiology Department, Central Leprosy training and Research institute, Chennai, India
  • Pradeep C S College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia
  • Bindu Rani S R Department of Microbiology and Biochemistry, Shanthidhama College of Nursing Sciences, RGUHS, Bangalore, Karnataka, 560091, India

DOI:

https://doi.org/10.38150/sajeb.2(6).p256-258

Abstract

The 16S rRNA methylases confer and cause panâ€aminoglycoside resistance (PAR). In the present prospective study, aminoglycosideâ€resistant isolates were subjected to the disc diffusion and agar dilution methods to determine minimum inhibitory concentrations (MICs) to rule out highâ€level aminoglycoside resistance (HLAR). One hundred and two isolates had an MIC ≥ 512 μg/ mL. These 102 isolates were investigated for the presence of 16S rRNA methylase genes including armA, rmtA, rmtB, and rmtC by polymerase chain reaction (PCR). Among isolates belonging to Enterobacteriaceae, armA, rmtB, and rmtC were present in 28, 56, and 4 isolates, respectively. Meanwhile, armA, rmtA, rmtB, and rmtC were present in 6, 1, 4, and 2 nonâ€fermenting Gramâ€negative bacilli (NFGNB) isolates, respectively. All Enterobacteriaceae isolates were extended spectrum betaâ€lactamase (ESBL) positive. The present results demonstrate the wide dissemination of PAR among NFGNB compared to Enterobacteriaceae. These results are very similar to those in other countries, in which 16s rRNA methylases are common in Enterobacteriaceae. The rapid spread of multiâ€drugâ€resistant isolates producing both ESBL and 16s rRNA methylases raises clinical concerns and may become a major therapeutic threat in the future.

Author Biographies

Lakshmana Gowda, College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

James John, Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, Tamil Nadu, 632004, India

Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, Tamil Nadu, 632004, India

Mohammed Ali M. Marie, College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

Sangeetha Gopalkrishnan, Microbiology Department, Central Leprosy training and Research institute, Chennai, India

Microbiology Department, Central Leprosy training and Research institute, Chennai, India

Pradeep C S, College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

College of Applied Medical Sciences, Clinical Laboratory Department, King Saud University, P. O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia

Bindu Rani S R, Department of Microbiology and Biochemistry, Shanthidhama College of Nursing Sciences, RGUHS, Bangalore, Karnataka, 560091, India

Department of Microbiology and Biochemistry, Shanthidhama College of Nursing Sciences, RGUHS, Bangalore, Karnataka, 560091, India

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Published

2013-01-23

Issue

Vol. 2 No. 6 (2012)

Section

Research Articles