Fluoride Induced Effect on Spleen of Rat: Light & Transmission Electron Microscopic Studies

Abstract

Fluoride, a strong electronegative anion has a disruptive effect on various tissues in the body. The rats (Rattus norvegicus) were fed with Sodium fluoride (NaF) at 25mg/kgâ€bw and 50mg/kgâ€bw daily for 60 days. The abdominal cavity was opened after cervical dislocation of rats and spleen was dissected out. The organs were cut into pieces and processed for light and transmission electron microscopy. Animals treated with fluoride showed the degenerative changes in the morphology of spleen. The photomicrographs showed that the capsule and trabeculae were broken at many places, many large spaces occured in between the concentric array of cells in follicles of white pulp and the cells of PALS infiltered in the red pulp area. The electron micrographs revealed the abnormal shaped nucleus with swollen and enlarged mitochondria and degenerated cristae in the cytosol. The above results suggests that the spleen treated with fluoride might loss or reduce the ability of contraction and adversely affect the immuogenicity by not synthesizing the ATP required during the clonal expression of Bâ€cells.