Green Synthesis and Characterization of Silver Nanoparticle Using Methanolic Extract of Ventilago madraspatana Gaertn. Leaves

Abstract

A growing number of researchers are exploring the eco-friendly production of silver nanoparticles (AgNPs) and their potential uses, particularly in targeted tissue therapy. This study aims to produce and analyze AgNPs derived from the leaves of Ventilago madraspatana, using a green synthesis method. Silver nitrate was dissolved in deionized water to obtain a 1 mM solution of AgNO3, and the methanolic crude extract was mixed with the silver nitrate solution under specific conditions. The formation of silver nanoparticles was confirmed by the appearance of a yellowish-brown color. The synthesized silver nanoparticles were comprehensively characterized to elucidate their properties using a range of techniques, including UV-Vis spectroscopy, FTIR, XRD, SEM and TEM. A strong, shiftless absorption band was observed at 420 nm via UV-Vis spectroscopy, attributable to the surface plasmon resonance of AgNPs, confirming the successful synthesis of nanoparticles. FTIR spectroscopy revealed significant bands corresponding to functional groups. The morphology of the synthesized nanoparticles was characterized using SEM and the nanoparticles were found to be spherical, with a size range of 78-92 nm. This was confirmed by TEM, which showed that the nanoparticles were predominantly spherical, with a size range of 51 nm at 50 kx magnification. XRD analysis confirmed the crystalline structure of the synthesized silver nanoparticles. This study demonstrates the successful synthesis of silver nanoparticles using the methanolic extract of V. madraspatana and determination of their structure, providing a basis for their use in a wide range of biomedical and industrial field.