Antifungal activity of Olea europaea subsp. sylvestris tar against Fusari-um oxysporum f. sp. albedinis, the causal agent of Bayoud of the date palm in Southwest Algeria

Authors

  • Khadidja Bendjima Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria
  • Ahmed Makhloufi Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria
  • Amina Mezouari Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria
  • Khadidja Makhloufi Department of Electrical Engineering, Faculty of Technology, Laboratory of Control Analysis and Optimization of Electric Systems

DOI:

https://doi.org/10.38150/sajeb.10(2).p90-94

Abstract

Bayoud, a vascular disease of date palm, caused by Fusarium oxysporum f. sp. albedinis (FOA) is the most damaging disease of date palm in Algeria, with no effective control strategy yet available. This research aims to esti-mate the antifusarium activity of the Olea europaea subsp. sylvestris tar growing in Bechar (Algeria) against FOA, the cause of Bayoud disease of the date palm. The values of physicochemical analysis of the tar such as density value, index of refraction, pH and dry matter percentage were also deter-mined. In order to identify FOA pathogenic isolates, a specific PCR assay was used to verify that the isolated strains were the Bayoud pathogen FOA. Tar extracted from O. europaea subsp. sylvestris was tested for its antifungal activity against FOA. The physicochemical analysis results of vegetable tar showed a density value of 1.08, an index of refraction 1.5110, a pH of about 3,7 and 35% of dry matter. The specific PCR analysis allowed absolutely that all the isolates corresponded well to the Bayoud pathogen. The results of the antifungal activity revealed that the O. europaea subsp. sylvestris tar has a great percentage of inhibition against FOA, with minimal inhibition concen-tration value 2,1 μg/ml. This confirms the possible implication of vegetable tar in triggering date palm defence against its pathogen and confers a prom-ising strategy for effective control of the bayoud disease. 

Author Biographies

Khadidja Bendjima, Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria

Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria 

Ahmed Makhloufi, Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria

Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria 

Amina Mezouari, Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria

Laboratory of Valorization of Vegetal Resource and Food Security in Semi-Arid Areas, South West of Algeria, Tahri Mohammed University of Bechar, Algeria 

Khadidja Makhloufi, Department of Electrical Engineering, Faculty of Technology, Laboratory of Control Analysis and Optimization of Electric Systems

 

Department of Electrical Engineering, Faculty of Technology, Laboratory of Control Analysis and Optimization of Electric Systems, 

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Published

2020-04-19

Issue

Vol. 10 No. 2 (2020)

Section

Research Articles