Evaluation of leaf extracts from Algerian Argania spinosa. L. Skeels. (Sapotaceae) trees: Total phenol content, protein content, and Antibacterial activities against six against clinical phytopathogenic bacteria

Abstract

Argania spinosa (L.) Skeels. (Argan) is tropical species, this plant is known bythese therapeutic and medecinal uses. For this purpose, we test antibacterial potential of leaf extracts of three Algerian population of Argan trees andwe evaluate the geographical distribution and solvent extraction effect onthe antibacterial activities. In first, Levels of total phenol content, total protein content of leaf extracts were determined by UV-spectrophotometer,secondary, The agar well diffusion method were used to determine enzymeactivities of proteins, antibacterial effect and the minimal inhibitory concentration (MIC) of extracts. The amounts of the phenol and protein concentrations in tested extracts were in ranged respectively between (118.83-147.17mg/mL) of Gallic acid equivalents (mg/mL) ) and (31.33-40.55mg/mL)of BSA (mg/mL), the crude fresh extract and crude protein extract showperoxidase activities, while protease activities were not found in ours extracts. Microbiological tests showed a high antibacterial potential of methanol extracts against tested bacteria ,Enterococcus faecalis (ATCC 29212) wasmost sensitive to methanol extracts (IZD: 17mm to 23.5),in opposite, aqueous extracts show weak antibacterial activities. Crude protein extracts hadnot antibacterial effects while crude fresh extracts were efficiency againstbacterial growth, biochemical enzyme tests revealed that crude extracts ofthe leaves show peroxidase activity wish may to increase antibacterial activities of crude extracts. The high activities of leaf Argan extracts were detected in Oran samples (littoral area) followed by Tindouf samples (arid area),this result confirmed positive relationships between geographical distribution of samples and their antibacterial activities.