GC-MS and LC-MS based phytochemical profiling and quantification of Mangiferin in six species of Salacia from the Western Ghats of India

Abstract

The Salacia is a genus of plants in the family Celastraceae belongs to the ma-jor group Angiosperms. Several species in this genus have been used in tradi-tional medicine. Very limited phytochemical studies have been reported so far in Salacia from Western Ghats of Karnataka. Hence the aim of the pre-sent study was to screen the phytochemical compounds and quantitative analysis of mangiferin content in six species of Salacia. Which were found to be adequate to evaluate the raw material and can also be used as reference standards for the quality control/quality assurance purposes. The quantita-tive profiling of phytochemicals were analysed using Gas Chromatography-Mass Spectrometry and the concentration of mangiferin compound present in stem and root samples were quantified using High performance liquid chromatography method. GC-MS analysis revealed the presence of a vast array of phytoconstituents such as fatty acids, saturated hydrocarbons, het-erocyclic compounds associated with different plant parts. HPLC analysis has shown the presence of mangiferin in all the six species of Salacia, whereas S. chinensis root extract showed highest quantity of mangiferin. S. malabarica, S. oblonga and S. gambleana leaf extract contains the important unsaturated fatty acids and vitamin d-alpha tocopherol which have proven to attenuate oxidative stress and having anti-inflammatory effects. It can be noted that S. chinensis and S. oblonga extract contains higher level of poly unsaturated phytochemical compounds. The study identified that S. chinensis and S. ob-longa could be the most promising raw material for anti-diabetic drug formu-lations based on the phytoconstituent and mangiferin content.