Antibacterial activity of Lactobacilli detected in Algerian raw honeys against gram-negative bacteria

Authors

  • Homrani Mounia Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis Universi- ty, 27000 Mostaganem, Algeria
  • Dalache Fatiha Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria
  • Bouzouina Mohammed Plant Protec on Laboratory, Abdelhamid Ibn Badis University, Department of Agronomy, 27000, Mostaganem, Algerie
  • Nemmiche Said Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria
  • Homrani AbdelKader Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

DOI:

https://doi.org/10.38150/sajeb.8(3).p83-90

Abstract

This study reports the isola on of Lactobacilli from Four (n=04) typical Apis Mellifeca raw honey samples collected from different regions in Algeria and the evalua on of their an bacterial ac vi es against Gram-negative bacteria. The isolation of Lactobacilli was performed using different media. Isolates were identified  firrstly by catalase test, Gram staining and cells morphology, and confirmed by MALDI-TOF-MS. An bacterial activities of Bacterial cultures of selected Lactobacilli and their cell free supernatants were tested by agar spot assays and agar well diffusion assay. A total of eighteen (n=18) bacteria isolated from honey samples were presump vely iden ed as Lac- tobacillus spp. based on their posi ve Gram reactions, absence of catalase and their rod shape. MALDI-TOF MS iden ca on confirmed that all isolates were classified as Lactobacillus genus, but the results for their species were inconclusive with uncertainty between, Lactobacillus pentosus, Lactobacillus plantarum and Lactobacillus paraplantarum. Isolates exhibited an microbial activity with inhibition zone diameters ranging from 11 ± 1.41 mm to 17.5 ± 0.71 mm. 6 on 11 Lactobacilli Supernatants demonstrated inhibitory ac vity against all target bacteria. This study reveals the existence of Lactobacilli in Algerian raw Honeys. These Lactobacilli possess an bacterial properties against Gram-negative bacteria, often responsible of human infections, and can be a favorable substitute to antibiotics.

Author Biographies

Homrani Mounia, Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis Universi- ty, 27000 Mostaganem, Algeria

Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

Dalache Fatiha, Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

Bouzouina Mohammed, Plant Protec on Laboratory, Abdelhamid Ibn Badis University, Department of Agronomy, 27000, Mostaganem, Algerie

Plant Protec on Laboratory, Abdelhamid Ibn Badis University, Department of Agronomy, 27000, Mostaganem, Algerie

Nemmiche Said, Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

Homrani AbdelKader, Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

Laboratory of Sciences and Technics of Animal Production, Department of Agronomy, Abdelhamid Ibn Badis University, 27000 Mostaganem, Algeria

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Published

2019-03-10

Issue

Vol. 8 No. 3 (2018)

Section

Research Articles