Physicochemical Characterization and In-Vitro Antimicrobial Activity of Chitosan Extracted from Shrimp Shells Waste from Beni Saf Sea, Algeria

Abstract

Chitosan is the most abundant natural organic polymer in nature. Its positive charge and its molecular arrangement confer interesting properties on the plane food, pharmaceutical, medical, cosmetic, water treatment. The present study was undertaken to study the physiochemical parameters and the in vitro antimicrobial activity of chitosan extracted from shrimp shells waste. The molecular weight of chitosan is 1414.33±16.99 kDa with, the percent of Ash 0,345±0,040 %, moisture is 2,98 ±0,13 % , and protein is 0.3 ±0,041 %.Chitosan produced (5 %) was also characterized with Fourier Transform Infrared Spectroscopy (FTIR) the spectrum of the chitosan sample from the shell recorded 16 peaks in the range of 689.40/cm and 3430.02 /cm. The antibacterial and antifungul activities of chitosan were examined against Escherichia coli ATCC10536, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC7644, Staphylococcus aureus ATCC29213, Aspergillus niger ATCC 16888 and Candida albicans ATCC 10231 by agar wells diffusion the tests inhibitions zones diameters were 49,74± 0,75 , 54,35±0,93 , 42,27±1,07 , 32,95±0,28 and 53,73±0,64mm respectively. The minimum inhibitory concentration (MIC) using a broth microdilution method against tested microorganisms was ranging from 008% to 1.22%. These results open interesting perspectives of the chitosan. It can be used as new biomaterial with utility in many industrial areas.