Induction of Humoral Immune Response by 66 kDa Protein and Amplification of Gene present by using Polymerase Chain Reaction

Abstract

Malaria is one of the most dreaded vector borne disease caused by apicomplexan parasite, of the genus Plasmodium. In the present study the gene which encodes 66kDa protein in rodent malaria parasite, Plasmodium berghei, was cloned. Rodent malaria parasite, Plasmodium berghei (NK-65), was found lethal to white Swiss mice (BALB/c). The immunogenicity and protective efficacy of purified 66 kDa protein was evaluated both in vitro and in vivo. Mice immunized with 66 kDa protein induced a strong humoral immune response, revealing role of 66 kDa in malaria control. Genomic DNA of P. berghei was isolated from cell free parasite. Genomic DNA serves as a template for isola on of gene encoding 66 kDa protein. Further, the gene was amplified by using polymerase chain reaction. The amplified DNA fragment of 1717 bp was extracted from agarose gel and puri ed. The purified gene was ligated to a cloning vector for cloning procedure by TA-cloning mechanism. Results of this study provide evidence that 66 kDa protein is highly immunogenic during experimental infec on in rodents and such an gen will be a suitable candidate malaria vaccine.