Studies on Bacillus SVTH6 Isolated from Leachate of Banaskantha District in Gujarat, India

Abstract

Industrial development in context with metal mining industries has received increasing importance now-a-days due to increased metal demand. With the concern of environment mining activities should be ecofriendly and economical. Bioleaching a biohydrometallurgical dissolution process, in which bacteria are used to leach out metals from ore. In bioleaching indigenous microbes either acidophilic, autotrophic, heterotrophic, or mixotrophic play important role. Bacteria of genera Acidithiobacillus, Leptospirillum, Sulfolobus, Sulfobacillus, Delftia sp., Oxalobacter sp., Bacillus sp. and Ferribacter sp. have been isolated from mining environment. In the study presented here thiosulphate utilizing Bacillus SVTH6 is used which was isolated from leachate sample of mining site. The organism was studied for thiosulphate utilization; cultural, morphological, cellular and biochemical characteristics; sugar utilization profile as well as antibiotic susceptibility. Growth optimization study of Bacillus SVTH6 at static and shaking condition as well as different pH and NaCl concentration(s) will be disussed in detail.