Unmethylated/hypomethylated status of developmentally important genes in brain tissue of mammals

Authors

  • Manisha Sachan Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad-211004, India
  • Rajiva Raman Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221005, India

DOI:

https://doi.org/10.38150/sajeb.4(5).p226-231

Abstract

 

We have studied the de novo DNA methylation of 16 CpGs in the non-CpG island coding region of the proto-oncogene c-fos, 26 contiguous CpG in regu-latory region of HoxB5 and 27 CpGs in c-mos. This analysis has been done in brain tissue of mammals in fetal, neonatal and adult stages of development. The unmethylated/partial methylation of all CpGs in all three genes suggests that there could be tissue-specific hypomethylation of genes in brain or hy-pomethylation is a more common feature of specific genes in brain regard-less of their expression.

Author Biographies

Manisha Sachan, Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad-211004, India

 

Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad-211004, India

Rajiva Raman, Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221005, India

 

Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221005, India

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Published

2015-01-20

Issue

Vol. 4 No. 5 (2014)

Section

Research Articles