In vivo stimulatory effects of wheat bran on intestinal microbial ecosystem of mice

Abstract

The prebiotic effect in vivo of wheat bran on growth and colonization of both strains bifidobacteria (Bifidobacterium animalis subsp lactis Bb12 and B. bifi-dum Bb 443) and two lactic strains (Lactobacillus acidophilus LbA - CEC-T4529; L.rhamnosus LBRE - LSAS) was investigated. Conventional Swiss mice received a daily dose of orogastric monocultures of different strains tested for 4 weeks. These mice were fed a diet supplemented with or without wheat bran by weight. Changes in weight, implantation of strains and modifi-cation of microflora were monitored throughout the study. At the end of the experiment, after 4 weeks of observation, the sample of blood, various or-gans, and tissues of the digestive tract were collected to determine the he-matological parameters and biochemical parameters, blood and various or-gans were used in the study of bacterial translocation and gut tissue in the histological study. The results show the prebiotic effect of wheat bran on the location of the strains studied. This colonization by beneficial bacteria con-tributes significantly to the modification of the intestinal microbiota. Indeed, a reduction rate of the germ Bacteroides was recorded. The results of hema-tological and biochemical parameters showed no significant difference be-tween mice of different lots. In general, the values were within the stand-ards. According to the histological study of vital tissues, administration of lactic three strains (Bb12, Bb443 and LBRE- LSAS) has revealed no expression of inflammation or necrosis. In contrast, inflammatory effects and tissue ne-crosis were detected during the administration of Lactobacillus acidophilus strain.