HAT-RAPD profiling of seven fungi isolated from central Kazakhstan soils

Konstantin Li; Assel Moldagulova; Eleonora Bekenova; Abzal Kazhibaev; Markhabbat Kairova; Maira Urazova; Serik Shaikhin; Kairtay Almagambetov

doi:10.38150/sajeb.4(1).p17-23

Authors

Konstantin Li Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Assel Moldagulova Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Eleonora Bekenova Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Abzal Kazhibaev Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Markhabbat Kairova Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Maira Urazova Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Serik Shaikhin Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000
Kairtay Almagambetov Republic Collection of Microorganisms (RCM) SC MES, Valikhanov st.13/1, Astana, Republic of Kazakhstan, 010000

DOI:

https://doi.org/10.38150/sajeb.4(1).p17-23

Abstract

Stable HAT-RAPD (high annealing temperature) profiles were obtained for 7 micromycetes isolated from the central Kazakhstan soils with single non-specific primers. An arbitrary primer (decamer AP3), a primer derived from the intergenic spacer regions (T3B) and microsatellite primer (GTG)5 were applied in PCR. Precise and reproducible profiles of the amplification prod-ucts were obtained for all seven hyphomycetes isolates in spite of increased annealing temperature application. The number and size of amplicons reflect a species specificity. In some cases intraspecific variability was observed. Cluster analysis of the profiles demonstrated the phylogenetic relationships between isolates that are consistent with those of ITS-region genotyping. Obtained results completely justify the application of the HAT-RAPD in mark-ing and rapid identification of soil hyphomycetes.