Study of Antinflammatory properties of Lactic Acid Bacteria in experi-mentally induced musculoskeletal symptoms in Wistar Rat

Abstract

Rheumatoid Arthritis (RA) is a manifestation of body from multiple causes of weakened immune system, a developed internal allergic response to un-known allergens, hormonal factors, stress and other unknown factors. The present study was undertaken to determine the Anti-arthritic and Anti-inflammatory property of Lactobacillus acidophilus as probiotics against Col-lagen induced arthritis (CIA) in wistar rat. CIA (100μl) was induced intrader-mally at several sites on the back. A dose of L. acidophilus 2 x 108 CFU/ml was administered orally after induction of arthritis i.e. on 7th day. Dexame-thasone was given as standard drug in the dose of 0.3-3.0 mg/kg body weight. Paw thickness was measured and scored regularly. After sacrifice, blood was withdrawn for haematological, biochemical and serological analy-sis. Histopathology of liver, kidney and ovary was also performed. Oral ad-ministration of Lactobacillus significantly decreased the serum ceruloplasmin level. Beneficial effects such as increased adhesive property shown by Lactic Acid Bacteria (LAB), increased haemoglobin content and RBC and decreased WBC counts were observed. High level of C’ Reactive Protein was observed in arthritic control rats. However, L. acidophilus treatment significantly de-creased CRP level. Standard Drug also reduced the CRP level. Similarly Rheu-matoid factor was also decreased in LAB and standard drug group as com-pared to the control. In conclusion LAB has potent anti-arthritic property against and shown reduced level of RF and CRP in collagen induced arthritis in wistar rats.