Comparative studies of viability of clinical Escherichia coli and Escherichia coli ATCC 25922 under different preservation methods

Authors

  • A. N. Sulaiman Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • J. B. Ameh Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
  • H. I. Inabo Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

DOI:

https://doi.org/10.38150/sajeb.3(3).p119-126

Abstract

In this study, four methods of bacterial preservation: (sterile soil, sterile distilled water, agar overlaid with mineral oil and lyophilization) were employed for maintaining clinical Escherichia coli and Escherichia coli ATCC 25922 for a period of 180 days. The morphology, Gram’s staining properties, biochemical characteristics and viability were monitored at regular intervals of 30 days for 180 days. The spread plate colony count method was used to investigate the rate of recovery or viability of all the two organisms from equally inoculated bacterial suspensions (2.0 McFarland equivalent to 8.8 log10 cfu/ml) in differently prepared stock cultures of each preservation method. Agar overlaid with mineral oil was found to be the best method for maintenance and preservation of both organisms.

Author Biographies

A. N. Sulaiman, Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

J. B. Ameh, Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

H. I. Inabo, Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria

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Published

2013-08-15

Issue

Vol. 3 No. 3 (2013)

Section

Research Articles