South Asian Journal of Experimental Biology, Vol 1, No 4 (2011)

Jul 15
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Induction of Colon tumor and Intestinal tumor in Swiss Albino Mice by the DihydroMicrocystin –LR, [D‐Asp3] Microcystin‐WR and [D‐Asp3] Microcystin‐HtyR produced by the Cyanobacterial strains Isolated from water bodies in semi‐desert region of India

R. Ashwin Kumar, K. Ramani, S. K. Verma


The crude extracts of two cyanobacterial strains, isolated from water bodies

of Shekhawati region of India, and identified as


Anabaena doliolum and

Nostoc spongiaeforme,



in‐vivo mouse bioassay model. A dose of 100μg of total

protein/kg of body weight showed restlessness, spasmodic leaping, slow

movement and loss of co


‐ordination within 6hours of intraperitonial (i.p)

administration in Swiss albino mice. Further purification of crude extracts

with the help of thin layer chromatography using silica gel GF


60 plates,

showed four spots for each of these strains. The chronic toxicity studies with

sub lethal dose of spot


‐3 from A. doliolum have shown to cause colon tumors,

whereas sub lethal dose of spot


‐3 from N. spongiaeforme induced the

intestinal tumors. An increase in oxidative stress (MDA level), serum ALT,

ALP, AST and LDH level was also found in both the cases. These spots were

further analysis with HPLC and MALDI TOFF Ms and were identified as Dihydro

Microcystin –LR and [D


‐Asp3] Microcystin‐WR in case of Ad spot‐3 and



‐LR and [D‐Asp3] microcystin‐HtyR in case of Ns spot‐3.

showed high level of hepatotoxicity and tumor promoting

activity in


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