South Asian Journal of Experimental Biology, Vol 3, No 4 (2013)

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Feb 26
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Assessment of a Loop‐Mediated Isothermal Amplification Assay targeting lytA genes with conventional PCR for the direct detection of Streptococcus pneumoniae in clinical samples

Mohammed Ali Marie, Pradeep C. S., James John, Sanggeetha Gopalkrishnan, Lakshmana Gowda K., Khaled Homoud M. Dabwan

Abstract


Streptococcus pneumoniae is one of the most encountered pathogens in developed and developing countries. It is a leading cause of invasive bacterial disease in adults as well as in children. This study focuses on the loop-mediated isothermal amplification (LAMP) assay to validate its suitability for directly detecting lyt A target genes of S. pneumoniae in clinical samples. We studied the clinical sensitivity and specificity of the LAMP assay targeting lyt A using 42 selected CSF specimens from children with suspected meningitis in the Kingdom of Saudi Arabia. Conventional polymerase chain reaction (PCR) and culture tests were also performed. The detection rate of the LAMP assay was significantly higher than the rates of PCR and culture tests and the detection limits (10 copies by LAMP) were considerably lower than those for PCR (103 copies). Our study suggests that LAMP reaction-based detection of target genes of suspected pathogens could be applied in a various clinical settings. In addition, the lower cost of LAMP assay than PCR makes it more economical, allowing its use in laboratories with limited resources.

 


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