South Asian Journal of Experimental Biology, Vol 2, No 6 (2012)

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Mar 29
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Detection of Pan‐amino glycoside‐resistant Gram negative Bacteria using a Molecular Method

Lakshmana Gowda, James John, Mohammed Ali M. Marie, Sangeetha Gopalkrishnan, Pradeep C S, Bindu Rani S R

Abstract


The 16S rRNA methylases confer and cause pan‐aminoglycoside resistance (PAR). In the present prospective study, aminoglycoside‐resistant isolates were subjected to the disc diffusion and agar dilution methods to determine minimum inhibitory concentrations (MICs) to rule out high‐level aminoglycoside resistance (HLAR). One hundred and two isolates had an MIC ≥ 512 μg/ mL. These 102 isolates were investigated for the presence of 16S rRNA methylase genes including armA, rmtA, rmtB, and rmtC by polymerase chain reaction (PCR). Among isolates belonging to Enterobacteriaceae, armA, rmtB, and rmtC were present in 28, 56, and 4 isolates, respectively. Meanwhile, armA, rmtA, rmtB, and rmtC were present in 6, 1, 4, and 2 non‐fermenting Gram‐negative bacilli (NFGNB) isolates, respectively. All Enterobacteriaceae isolates were extended spectrum beta‐lactamase (ESBL) positive. The present results demonstrate the wide dissemination of PAR among NFGNB compared to Enterobacteriaceae. These results are very similar to those in other countries, in which 16s rRNA methylases are common in Enterobacteriaceae. The rapid spread of multi‐drug‐resistant isolates producing both ESBL and 16s rRNA methylases raises clinical concerns and may become a major therapeutic threat in the future.

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